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In addition to the original genotype 1b isolate, called Con1, replicons have now been established for other 1b isolates and for genotypes 1a and 2a, and HCV RNA replication has been achieved in Hela, 293, HepG2 and even mouse hepatoma cell lines 13

Its also recycled and rebuilt, which means it depends on adequate building blocks and overall metabolic steadiness, not just a one-time input

The I7205, 14-13, FK1 antibodies were previously validated in similar studies using no-infection controls or controls with no ubiquitylation 45