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On the other hand, the gene expression of mitochondrial biogenesis markers ( PGC1 , AMPK , SIRT1 , NRF2 , and MFN2 ) and mitochondrial activity marker ( COXIV ) in liver-specific GHRKO (LiGHRKO) and wild-type mice are significantly different between the males and females, suggesting that sexual dimorphism may also play an essential role in regulating the mitochondrial biogenesis (Zawada et al., 2015)

We are constantly exposed to toxins that our liver must detoxify - using up our glutathione