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Decreases in NAD+ levels have been associated with reduced neuronal signaling and impaired neuromuscular communication in animal models

Cell viability assay Cell proliferation was detected via a CCK-8 assay (Biosharp, China) or a CellTiter-Glo assay (Promega) according to the manufacturers instructions

Ethics declarations Ethics approval and consent to participate This study protocol was approved by the Ethics Committee of Shiraz University of Medical Sciences and performed in accordance with the Ethical Standards laid down in the 1964 Declaration of Helsinki and its later amendments

doi: 10.1038/s41392-023-01629-8 127 PenningerJ