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Cell culture Clonal insulin-secreting BRIN-BD11 cells were maintained in RPMI 1640 medium containing 11.1 mM glucose, supplemented with 10% foetal bovine serum (FBS), 100 U/ml penicillin and 0.1 mg/ml streptomycin, pH 7.4, as described previously 45

The available scientific information in relation to the quantification of BDNF isoforms in human samples is scarce, and the studies have primarily focused on populations with neurological problems due to the added value of BDNF as a biomarker in these pathologies (Supplementary Table S1)

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The evidence is diverse and includes extensive work with laboratory animals, otherwise unexplained associations, factors associated with APAP metabolism, and limited studies in humans