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-H2AX plays an essential role in the recruitment and accumulation of DNA repair proteins at sites of double-strand break (DSB) damage due to exposure to DNA-damaging compounds (PAHs and acrylamide), such as those found in the HnB mainstream smoke ( Next, we investigated the effects of HnB exposure on testis antioxidant response and our results indicate activation of NRF2-dependent machinery, including catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GSSG-Red), and NADPH quinone reductase (NQO1), whereas no significant changes were observed for glutathione peroxidase (GSH-Px) in testicular tissue

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