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The EC 50 value was derived from technical triplicates, and standard deviation reflects two individual experiments conducted on separate dates using freshly isolated T cell isolation from the same donors frozen PBMCs

Clinical studies have supported the use of AOD-9604 for weight loss in animal subjects, and while some human trials have shown promising results for fat burning, the extent of its effectiveness can vary among individuals

Mitochondrial number was also dose dependent, but interestingly not variable by time or glutathione supplementation, suggesting a rapid and oxidative stress-independent mechanism of mitochondrial damage by SP-2509 and SP-2577 (Figure 3C, q = n.s.)

In: Azar FM, Beaty JH, eds