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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing

Holst, Wewer Albrechtsen, Gabe, Rosenkilde)

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[10] [11] These changes in fluorescence are also used to measure changes in the redox state of living cells, through fluorescence microscopy