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Compounds 1 and 2 showed the most potent COX-2 activity and selectivity (Figure 6)

the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

Vitamin, mineral, polyphenol, amino acid profile of bee pollen from Rhododendron ponticum (source of mad honey): nutritional and palynological approach

Limitations of the Literature The Panel proceeded with full awareness of the limitations of the IC/BPS literature, which include: poorly-defined patient groups or heterogeneous groups