The cohort included 82% males and 18% females, and children with genetic syndromes, prematurity, major medical disease or severe irritability were excluded, although common comorbidities such as gastrointestinal, allergic, neurological, and sleep disorders were present
After 4 h, the resulting product was collected by centrifugation and refluxed in a 1.5% ammonium nitrate solution for 12 h to remove the CTAT template

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High doses (1,000+ mg/day) are often needed for minimal effects