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21,25 Retatrutide, a triple hormone-receptor agonist (GLP1, GIP, and glucagon receptors), has demonstrated high potential for weight loss in phase 2 studies of patients with obesity (up to 24% body weight loss at 48 weeks)

The obtained good 2D classes were used for template-based automatic particle picking, and false positives or bad particles were subsequently eliminated over two rounds of 2D classification, resulting in 9101 selected particles

The lipid extracts were cleared by centrifugation (17,000 g , 20 C, 10 min), and the lipids in the supernatant were analyzed by LC-MS